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. 2021 May 29;8:100099. doi: 10.1016/j.jvacx.2021.100099

Fig. 3.

Fig. 3

The binding in ELISA of multimer VHHs to TeNT both monospecific and bispecific in combination with dog IgG or albumin. Monospecific VHH binding to TeNT was analyzed both by TeNT – ganglioside inhibition ELISA and on plates coated with TeNT. In addition, bispecific binding requiring VHH binding to coated dog albumin (A12 containing multimeric VHHs) or dog IgG (G13 containing multimeric VHHs) and capture of soluble biotinylated TeNT is shown. The reciprocal of the EC or IC value of each VHH is shown. The EC values were interpolated at the indicated A450 values.