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Blocking by VHH3s of TeNT binding of 3 monomeric VHHs or the same VHH3 that was used for blocking. In biolayer interferometry experiments sensors were loaded with biotinylated TeNT and subsequently with blocking VHH3s. Then sensors were loaded with monomeric analyte VHHs or the same VHH3 as used for blocking. The percentage inhibition of the association signal of analyte VHHs as compared to a control assay without prior blocking by VHH3s is given.
