Supplemental Figure S2.

A: Same analysis as in Figure 2D is performed for the pilot data. The percentage of reads mapping to every target miR, both mature and isomiR, is plotted for each sample, comparing blocked and unblocked percentages B: Both mature and isomiR forms of miR-25 are retained in the count matrix rather than collapsing together. Pairwise differential expression (DE) is performed, and the top 50 most significantly differentially expressed miRs are plotted on a heatmap. C: Off-target summary of DE from panel A. Log₂ fold change (log₂FC) and log₂ adjusted P value plotted for each miR identified as DE (adjusted P < 0.05 and absolute log₂FC > 1, with the size of points corresponding to mean raw counts of each species).