Skip to main content
. 2021 May 10;7(6):2192–2197. doi: 10.1021/acsbiomaterials.1c00183

Figure 3.

Figure 3

Gradient of peptide concentration. (A) Chemical reaction used to functionalize the soft carboxylated agarose hydrogel with maleimide and successive click reaction with a cysteine-terminated peptide. (B) Reaction kinetics of functionalized agarose with the CRGDS peptide. (C) Effect of the functionalization on the rheological properties is shown through the storage shear modulus of native soft carboxylated agarose and its derivatives. Scheme representing the (D) 2D and (F) 3D bioprinting experiment of graded peptide immobilization. (E and G) Soft-maleimide bioink prints with CRGDS-FITC. Color analysis showing the change in intensity across the printed (H) 2D object and (I) 3D object. Error bars are standard deviation for n = 3. Scale bars: (E) 10 mm, (G) 1 mm.