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. 2021 Jun 15;21:274. doi: 10.1186/s12870-021-03060-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression patterns of genes related to the isoflavonoid biosynthesis pathway. a Schematic representation of the isoflavonoid biosynthesis pathway in legumes. b Differentially expressed genes (DEGs) encoding proteins related to isoflavonoid biosynthesis in control and PvRbohB-RNAi P. vulgaris roots at 7 dpi with R. tropici. The color bars represent the log₂(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log₂FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. PAL: phenylalanine ammonia-lyase; C4H: cinnamate 4-hydroxylase; 4CL: 4-coumarate CoA-ligase; CHS: chalcone synthase; CHR: chalcone reductase; CHI: chalcone isomerase; IFS: isoflavone synthase; CYP81E: isoflavone/4'-methoxyisoflavone 2'-hydroxylase