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. 2021 Jun 16;3:43. doi: 10.1186/s42523-021-00104-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The mean (±SE) density of bacteria (per 1800 μm²) on S. virgatus eggshells at Day 0 and Day 25 of incubation in soil slurry. Bacteria were significantly less dense on dissected eggs than on oviposited eggs on both days (p < < 0.001). Counts were made via SEM by randomly selecting locations on the eggshell surface and measuring the density of bacteria at 2.5 k magnification