Fig. 5.

SNHG16 maintained cancer stem-like cell dynamic via activating TLR7. SHG44 and U251 cells were transfected with TLR7 or si-TLR7 and incubated with exosomes from CSCs transfected with SNHG16 or si-SNHG16, respectively. A, B qRT-PCR for mRNA expression of TLR7 in SHG44 and U251 cells. n = 4, *p < 0.05. C, D MTT for cell viability in SHG44 and U251 cells. n = 10, *p < 0.05. E, F Would healing assay for cell migration in SHG44 and U251 cells. Scale bar = 500 μm. n = 4, *p < 0.05. G, H Transwell assay for cell invasion in SHG44 and U251 cells. Scale bar = 200 μm. n = 4, *p < 0.05. I, J Colony formation assay for cell proliferation in SHG44 and U251 cells. Scale bar = 10 mm. n = 6, *p < 0.05