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CLSM image of JEG-3 cells after incubation with various MTX formulations for 24 h. Red and green fluorescence represent the JC-1 aggregates and JC-1 monomer, respectively (a). The scale bar is 100 μm. Bio-TEM evaluation of the destructive effect caused by MTX formulations on JEG-3 cells, blank culture medium as control (b). The red box area is enlarged on the right, the blue arrows indicate the normal mitochondria, and the yellow arrows indicate the atrophied mitochondria
