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. 2021 Jun 15;17:219. doi: 10.1186/s12917-021-02915-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — a: PCR amplification of 504 bp Babesia spp. 18S rRNA, Lane 1, 2 negative control, Lane 3, 4, 5: test samples M: 100 bp ladder, Lane 6: positive control. b PCR amplification of 270 bp Anaplasma marginale 16 s rRNA, Lane 1–4 test samples, M1: 100 bp ladder, M2: 50 bp ladder, Lane 5: positive control, Lane 6 negative control. c PCR amplification of 751 bp Theileria annulata Tams1 gene, Lane 1: positive control, M: 100 bp ladder, Lane 3,4,5,6,: test samples at gradient, Lane 7: negative control