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. 2021 Jun 16;40:196. doi: 10.1186/s13046-021-01984-w

Table 1.

ddPCR primers and probes information for this study

Oligos Annealing Temperature
GNA11-F CTTTCAGGATGGTGGATGT
GNA11-R ACATGATGGATGTCACGTTCT 58 °C
GNA11_A_Allele 5HEX/AC + CGC + TGG + CC/3IABkFQ
GNA11_C_Allele 56-FAM/AC + CGC + GGGCC/3IABkFQ
GNA11_T_Allele 56-FAM/AC + CGC + AGG + CC/3IABkFQ
GNAQ-F CTTGCAGAATGGTCGATGTAG
GNAQ-R GCGCTACTAGAAACATGATAGAG 60 °C
GNAQ_A_Allele 5HEX/CCT + T + T + G + GCCC/3IABkFQ
GNAQ_Q209L_T_Allele 56-FAM/CCT + T + A + G + GCCC/3IABkFQ
GNAQ_Q209P_C_Allele 56-FAM/ACCT+T + G + GGCC/3IABkFQ
PLCb4-F TAACAAACGGCAAATGAGTCG
PLCb4-R CAGCCAGCGTTCCAGAAA 55 °C
PLCB4_D630Y_G_allele 5HEX/CGA + GT + C + G + ATT + CC/3IABkFQ
PLCB4_D630Y_T_allele 56-FAM/CGA + GT + C + T + AT+TC + CA/3IABkFQ
CYSLTR2-F CCTTGTATGTCAACATGTACAGC
CYSLTR2-R GTGAACCATTGCCAGGAAAC 55 °C
CYSLTR2_T_allele 5HEX/TTC + C + T + GA + CCGT/3IABkFQ
CYSLTR2_A_allele 56-FAM/TTC + C + A + GA + CCGT/3IABkFQ