Fig. 4.

Reactive oxygen species protein expression. Western blot was performed on mouse cardiac tissue to assess protein expression of selected proteins involved in reactive oxygen species production and inflammation. Relative protein expression is shown in part a, and cropped representative immunoblot images are shown in part b. the vinculin loading control is shown for each separate western blot that is shown in the figure. Note that NFkB and TNFα were assessed using different regions of the same western blot membrane. Full-length blots are presented in Additional files 1, 2, 3, 4: Figs. 1–4 (n = 2, *p ≤ 0.05, **p ≤ 0.01)