Table 2. Drugs, doses of lamotrigine (LTG) or valproate (VPA).
Time between injection and collection, method of administration (MoA) and number of animals used at each age (n = number of litters); i.p. (intraperitoneal), i.v. (intravenous). Animal losses (not recorded here): One E19 mother from blood loss during cannulation. Three P4 animals not used (2 dose errors, one died before end of experiment). In the chronic valproate feed experiments approximately half of the fetuses died in utero.
| Radio-labelled
drug |
Doses of cold drug
(mg/Kg) |
Time
(min) |
MoA | E19
n |
P4
n |
Adult
n |
|
|---|---|---|---|---|---|---|---|
| VPA | LTG | ||||||
| 3H-dextran | - | - | 5 | i.v. | 6 (1) | 5 (2) | 3 |
| 3H-VPA | 10 | - | 30 | i.p. | 5 (2) | 4 | |
| 3H-VPA | 30 | - | 30 | i.p. | To dam: 8 (1)
To pup: 5 (1) |
3 (1) | 4 |
| - | 30 | - | 30 | i.p. | 4 (1) | 3 | |
| 3H-VPA | 100 | - | 15 | i.p. | 2 (1) | ||
| 3H-VPA | 100 | - | 30 | i.p. | To dam: 6 (1)
To pup: 5 (1) |
3 (1) | 3 |
| - | 100 | - | 30 | i.p. | 4 (1) | 3 | |
| 3H-VPA | 100 | - | 60 | i.p. | 2 (1) | ||
| 3H-VPA | 100 | - | 90 | i.p. | 2 (1) | ||
| 3H-VPA | 200 | - | 30 | i.p. | 3 (1) | 4 | |
| 3H-LTG | - | 6 | 30 | i.p. | To dam: 12 (1) | 3 (1) | 3 |
| 3H-LTG | - | 20 | 30 | i.p. | To dam: 5 (1)
To pup: 8 (2) |
3 (2) | 3 |
| 3H-LTG | - | 20 | 60 | i.p. | 2 (1) | ||
| 3H-LTG | - | 20 | 90 | i.p. | 2 (1) | ||
| 3H-LTG | - | 20 | 120 | i.p. | 2 (2) | ||
| 3H-LTG | - | 40 | 30 | i.p. | 3 (2) | ||
| 3H-VPA | 30 | 2 | 30 | i.p. | 3 (1) | ||
| 3H-VPA | 30 | 6 | 30 | i.p. | To dam: 10 (1) | 4 (2) | 4 |
| 3H-VPA | 30 | 12 | 30 | i.p. | 4 (1) | ||
| 3H-VPA | 30 | 20 | 30 | i.p. | 4 (1) | ||
| 3H-LTG | 30 | 6 | 30 | i.p. | 3 (1) | 4 | |
| 3H-VPA | Feed+ 20 g/kg | - | 30 | i.p. | To pup: 6 (1) | 4 (1) | 4 |