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. 2021 Jun 2;11:647559. doi: 10.3389/fonc.2021.647559

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Copyright © 2021 Jiang, Huang, Jiang, Hou, Tian, Li, Sun, Zhang, Zhang, Li, Li, Tong and Ma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression levels of G6PD and SDHA are up-regulated in NSCLC. (A) Scanned panorama of human NSCLC tissue stained for G6PD (FITC), SDHA (TEXAS RED) and nuclear DAPI, with the representative carcinoma region amplified. (B) Analysis procedure for expression level of target proteins: Valid nuclei, indicated as green circles, were recognized based on size and DAPI intensity; then staining positive cells were identified as indicated by red circles. (C) Presentation of G6PD and SDHA expression levels based on staining area and intensity in carcinoma versus para-carcinoma regions. The upper number and lower number in each chart respectively denoted staining positive cells and staining negative ones.