FIGURE 1.

Combined single-cell Illumina and Nanopore sequencing strategy. GFP+/− cells are pooled and sequenced on the Illumina and Nanopore platform. The Nanopore platform generates long cDNA sequencing reads that are used in barcode calling and estimating read error parameters. The Illumina data are used to estimate the total number of cells in sequencing and the represented cell barcodes. The simulated data are then used to parameterize a Bayesian model of barcode alignment features to discriminate correct versus false barcode assignments. This model is then used on the real data to assign cell barcodes to Nanopore reads. The GFP label and known NMD transcripts can be used to validate this assignment.