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Sensitivity and specificity of ScNapBar and Sicelore on 100 Illumina libraries with different levels of saturation. (A) Barcode assignment with UMI matches. (B) Barcode assignment without UMI matches (ScNapBar score >50). (C) Benchmark of the specificity and sensitivity of the Illumina library with 100% saturation. We compared the barcode assignments with ScNapBar score >1–99, and the assignments from Sicelore with UMI support are roughly equivalent to the ScNapBar score >90.
