Figure 4.

DigiWEST multiplex Western blot validated alterations in distinct signaling pathways. Plantar incision (INC, red bars) induced significant dysregulation of multiple members of ERK1/2 signaling, PKA/PKC signaling, and Akt signaling (***P < 0.001 vs Sham, grey bars; 3 mice were pooled/replicate, 4 biological replicates in total). Results are expressed as scatter bar plots showing the mean ± SEM and single values for each biological replicate (open circles). P-values: ***P < 0.0001, multiple t-tests without assuming a consistent SE. Legend: T, threonine; S, serine; P, denotes the phosphorylated form of the respective analyte; numbers behind T/S indicate the respective amino acid residue; kDa, kilo Dalton (as ERK1/2 isoforms are known to run at different molecular weights).⁶⁹