Figure 5.

Changes in proliferation, cell cycle, and survival in BY4741 yeast in the presence of DNP during exposure to doxorubicin – BY4741 yeast were grown in YPD and exposed to 0 or 100 μM doxorubicin and either 0, or 0.2 mM DNP for up to 12 hours. Cell density was measured by counting on a hemocytometer at 0, 6, and 12 hours. A) Values at each time point with the same letter are not significantly different (Tukey’s HSD p < 0.05; n = 3). In the presence of 100 μM doxorubicin (dotted lines), 0.2 mM DNP caused a significant reduction in dox induced growth arrest at 12 hours. B) Budding patterns at 12 hours exposure showed significant increases in Large and No-bud yeast in the absence of doxorubicin with or without 0.2 mM DNP (Tukey’s HSD P < 005; N = 3) and a nominal decrease in multi-bud yeast. In the presence of doxorubicin, 0.2 mM DNP showed the significantly lower increase in multi-bud yeast at 12 hours with a concomitant increase in small-bud populations. Same color bars (bud type) with the same letter are not significantly different between yeast strain and time point groups (Tukey’s HSD p < 0.05; n = 3). C) 100 μM Doxorubicin did not cause a significant decrease in plating efficiency (survival) at 12 hours exposure. There was significant difference between groups at 12 hours (ANOVA p < 0.0478); however, no differences were identified after correcting for multiple comparisons (Tukey’s p > 0.5). Bars with the same letter are not significantly different from other exposures at the same time point (Tukey’s HSD, p < 0.05; n = 3)