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. 2021 May 11;87(11):e03075-20. doi: 10.1128/AEM.03075-20

TABLE 1.

Comparison of n-caproate production via lactate chain elongation by continuously fed reactor microbiome

Substrate Reactor type No. of days pH Dominant microbiome RA (%) Max concn (g liter−1) Max production (g liter−1 day−1) Reference or source
Thin stillage + H2 1-Liter reactor (with electro chemical cells) 42 5.4∼5.7 Megasphaera elsdenii 57 2.08 0.58 39
l-Lactate 0.7-Liter AFa (with in-line extraction) 220 5.0 Acinetobacter sp. 62.9 e 3.03 31
Grass 100-ml reactor (with electrochemical cells) 30 5.5∼6.2 Clostridium IV (related to strain BS-1) 28 4.09 e 28
Acid whey 0.7-liter CSTRb (with in-line extraction) 90 5.0 Bacteroidales 21.7 e 1.68 8
Clostridiales 12.6
15× diluted food waste leachate + H2 6.0-liter leach-bed 100 Initial, 7.0d Clostridium 42 9.59 e 29
Acid whey 1.0-liter UASBc 400 5.5 Ruminococcaceae 20.5 10.4 3.20 32
Prevotellaceae 17.9
Liquor brewing wastewater 5.0-liter AF 485 6.5 Clostridium IV 25.5 3.59 10.9 33
Xylan + lactate 1.0-liter CSTR 148 5.5 Ruminiclostridium 5 42.3 e 3.60 49
10–15% (vol/vol) food waste 1.0-liter stirred tank reactor 45 6.0 Caproiciproducensf 32–72 5.4 1.81 27
Lactate 0.9-liter CSTR 194 5.0–6.5 Caproiciproducens 2.0–67.8 8.58 e 34
dl-Lactate 3.0-liter CSTR 780 6.0 Caproiciproducensg 53.9 33.7 11.5 This study
a

AF, anaerobic filter.

b

CSTR, continuously stirred tank reactor.

c

UASB, upflow anaerobic sludge blanket.

d

Only the initial pH of each cycle was controlled.

e

No related data given.

f

Caproiciproducens in this source, formerly Clostridium IV, showed 91 to 95% similarity to Caproiciproducens galactitolivorans.

g

Caproiciproducens related to Ruminococcaceae strain CPB6.