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. 2021 May 6;12(23):8218–8223. doi: 10.1039/d1sc01269j

Fig. 4. Quantification of the elongation yield for the PNA HCR. (A) Scheme and result of streptavidin bead fluorescence pulldown assay. Reaction conditions: pH 7 1× SSC, 0.02% tween-20, 15 pmol of BpegI, 1 μM (150 pmol) of pegH1 and Cy3pegH2, and 4 h of reaction. (B) Fluorescence microscopy analysis for the estimation of HCR elongation. Reaction conditions: pH 7 1× SSC, 0.02% tween-20, 10 pmol of FBpegI or BpegI was loaded, 1 μM (200 pmol) of pegH1 and Cy3pegH2, and 4 h of reaction. The elongation fold reflects the number of hairpins incorporated into the HCR product relative to the initiator (elongation fold = (H1 + H2)/I).

Fig. 4