TABLE 1.
Steps | Vol (ml) | Total protein (mg) | Purification fold | Yield (%) |
CFS | 2,000 | 880 | 1 | 100 |
70%(NH4)2SO4 | 30 | 14.40 | 1.83 | 3 |
DEAE-C | 30 | 15 | 1.76 | 3 |
RP-HPLC | 1 | 0.0029 | 151.72 | 0.05 |
Its total protein, yield percentage, and purification fold were calculated as compared to the initial starting cell-free supernatant.