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. 2021 Jun 1;12:656896. doi: 10.3389/fmicb.2021.656896

TABLE 1.

Purification scheme of the antimicrobial protein from P. peoriae IBSD35 culture broth (pH∼6.8 at 38°C).

Steps Vol (ml) Total protein (mg) Purification fold Yield (%)
CFS 2,000 880 1 100
70%(NH4)2SO4 30 14.40 1.83 3
DEAE-C 30 15 1.76 3
RP-HPLC 1 0.0029 151.72 0.05

Its total protein, yield percentage, and purification fold were calculated as compared to the initial starting cell-free supernatant.