Fig. 1. (a) A scheme illustrating the construction of a DNA origami scaffold (rec-Ori) assembled with multiple fluorophores modified with ODN-tag. In this study, two types of fluorophores were assembled on a DNA origami scaffold. To assemble an ODN-tag tethered to a fluorophore on the DNA scaffold, a complementary sequence to the ODN-tag (comp-ODN) extended from staple strands was hybridized with the ODN-tag tethered fluorophore. (b) Structures of a 6-carboxyfluorescein (CF) derivative modified with oligo DNA (CF-ODN1) and a 6-carboxytetramethylrhodamine (CR) derivative modified with oligo DNA (CR-ODN2). (c) An illustration of a DNA origami scaffold assembled with two types of fluorophores (e.g. rec-Ori-CF5/CR5).