Fig. 4. Identification of the internalized rec-CR5 treated with SN21-LK15 (rec-CR5/SN21-LK15). (a) An illustration of rec-CR5 treated with SN21-LK15. (b) Confocal microscopy images of HeLa cells treated with rec-CR5/SN21-LK15 (magenta), FITC-dextran (cyan), and LysoDR (green). The DIC image includes a scale bar (20 μm). (c) Expanded image of the square in the merged image of (b). The arrows indicate FITC-dextran, rec-CR5, or LysoDR colocalized in the same area (white arrows: FITC-dextran and rec-CR5, red arrows: FITC-dextran, rec-CR5 and LysoDR). The merged image (green: FITC-dextran, red: rec-CR5, blue: LysoDR) includes a scale bar (5 μm). (d) Time lapse images of rec-CR5 (magenta) and LysoDR (green) from 40 min to 100 min after addition of rec-CR5 complexed with SN21-LK15. The merged image (magenta: rec-CR5, green: LysoDR) includes a scale bar (5 μm). The full image is shown in Fig. S12.†.