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Efficiency evaluation of the GAPA pathway. (A) AcP concentrations produced from 2.5 mM E4P and 10 mM GALD after 2 h using different enzymes (DeoC 2 mg/ml, RpiB 2 mg/ml, AlsE 2 mg/ml, and/or Fpk 1 mg/ml). (B) Time profile of GALD and AcP concentration in reaction system containing 10 mM GALD, 2.5 mM E4P, 2 mg/ml DeoC, 2 mg/ml RpiB, 2 mg/ml AlsE, and 1 mg/ml Fpk.
