FIG 3.

The cytokine responses in Aim2^−/− and Nlrp3^−/− mice differ from those in wild-type mice following infection with the emrA1 mutant. Wild-type and Aim2^−/− mice (A) and wild-type and Nlrp3^−/− mice (B) (n = 3 per group/time point) infected intranasally with 1 × 10⁶ CFU of the emrA1 mutant were sacrificed at the indicated time points. Lungs were collected, homogenized, and assayed for the levels of IL-6, TNF-α, and IL-1β. Results are expressed as picograms per lung (means ± SEM). The P values were determined using one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001. The data shown are representative of results from two independent experiments.