FIG 2.
Detection of B. recurrentis spirochetemia in selected CC mouse lines by qPCR. DNA samples of blood collected from B. recurrentis A17-infected CC001 (A), CC019 (B), CC042 (C), CC046 (D), and CC058 (E) mice at days 1, 2, 3, 4, 6, 8, and 10 postchallenge were subjected to quantification of flaB and actB genes by qPCR. Standard dilutions and DNA samples were amplified in triplicate. The numbers of spirochetes were calculated as the ratio of flaB copies per 106 actin DNA. When day 1 and day 2 data were compared within each mouse line, the significant difference was only detected for CC001 mice (P < 0.05; paired t test). When means were compared between the five CC lines for each time point, the significant difference was only detected for day 2 between mice of CC001 and CC046, CC019 and CC046, CC042 and CC046, and CC046 and CC058 (P < 0.005; ANOVA).