FIG 3.
Detection of B. recurrentis spirochetemia in cross-bred CC058-CC046 F1 mice by dark-field microscopy and qPCR. (A) Daily plasma samples were examined by dark-field microscopy for 10 days postchallenge. There was no significant difference in spirochetemic levels between days 1 and 2 (P > 0.05; paired t test). (B) DNA samples of blood obtained at days 1, 2, 3, 4, 6, 8, and 10 postchallenge were subjected to qPCR. The numbers of spirochetes were calculated as the ratio of flaB copies per 106 actin DNA. The significant difference was only detected between days 2 and 3 and days 2 and 6 (P < 0.05; ANOVA).