FIG 4.
IgM seroconversion of CC mice upon infection with B. recurrentis. Western blotting was performed by using whole-cell lysates of B. recurrentis A17 (1 × 106 cells per lane) and individual plasma samples collected from B. recurrentis A17-infected mice of lines CC001 (A), CC019 (B), CC042 (C), CC046 (D), CC058 (E), and CC058-CC046 F1 (F) at days 4 (lanes 1, 4, and 7), 7 (lanes 2, 5, and 8), and 10 (lanes 3, 6, and 9) postchallenge. For each panel, lanes 1 to 3, 4 to 6, and 7 to 10, respectively, represent plasma samples from mouse 1, mouse 2, and mouse 3 of the respective line. Preimmune plasma had no signal and is not shown.