FIG 4.
Mouse serum antiadhesin and antitoxin IgG titers (log10) from three groups i.m. immunized with MecVax, CFA/I/II/IV MEFA protein, or toxoid fusion protein 3xSTaN12S-mnLTR192G/L211A to show antigenic compatibility of the two proteins in MecVax. Mice i.m. immunized with MecVax (white bars) or CFA/I/II/IV MEFA protein (gray bars) developed similar titers of IgG to CFA/I, CS1, CS2, CS3, CS4, and CS6 (but not CS5, to which mice immunized with MecVax developed higher IgG titers; P < 0.05). Mice i.m. immunized with MecVax (white bars) and mice i.m. immunized with the toxoid fusion protein (hatched bars) developed the same titers of anti-STa and anti-LT IgGs. The fourth group, mice i.m. immunized with PBS (black bars) as the control, is also included. Bars and error bars represent the mean IgG titers and standard deviations. *, P < 0.05.