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. 2021 Jun 16;89(7):e00579-20. doi: 10.1128/IAI.00579-20

FIG 3.

FIG 3

PdpC and PdpD are dispensable for T6SS-dependent Francisella virulence in G. mellonella. Survival curves represent three individual experiments over 5 days pooled together (n0 total = 30; n0 = 10 per experiment). State of G. mellonella larvae was monitored every 12 h. Pupating larvae were censored (vertical dashes). Error bars indicate standard error. Individual curves were compared with log rank (Mantel-Cox) test. P values above a Bonferroni-corrected threshold were considered nonsignificant (ns). Parental strain versus mutant curves were compared. Black survival curves, PBS-treated G. mellonella larvae; gray survival curves, G. mellonella infected with F. tularensis subsp. novicida U112 iglA-sfGFP (parental strain); blue survival curves, G. mellonella larvae infected with F. tularensis subsp. novicida U112 iglA-sfGFP ΔpdpB strain (T6SS-negative control); red survival curves, G. mellonella infected with F. tularensis subsp. novicida U112 iglA-sfGFP (A) ΔpdpC mutant; (B) ΔpdpD mutant; (C) ΔpdpC ΔpdpD mutant; (D) ΔanmK mutant; (E) ΔopiA mutant; (F) ΔopiB1–3 mutant.