FIG 2.

The overexpression of CT813-FLAG from C. trachomatis L2 results in decreased detectable IncA, CT223, and IncE. HeLa cells were infected with C. trachomatis L2 CT813-FLAG, CT226-FLAG, or CT483-FLAG transformed strains, or wild-type C. trachomatis L2, and either not induced or induced at 7 hpi (5 nM or 20 nM aTc). At 48 hpi, infected monolayers were lysed in 8 M urea supplemented with 1% SDS, 10 mM Tris-HCl (pH 7.4), 2.5% β-mercaptoethanol, and nuclease. Lysates were normalized for equal protein content, separated by SDS-PAGE, and transferred to PVDF to blot for chlamydial proteins, MOMP, IncA, CT223, and IncE. GAPDH was used as a loading control. These data are representative of three independent experiments.