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. 2021 Jun 16;16(6):e0253170. doi: 10.1371/journal.pone.0253170

Fig 1. Clofarabine can bind CD99 and inhibit ES cell growth as a prodrug.

Fig 1

(A) Thermal stability of CD99 protein was tested in the presence of clofarabine (CLF) by western blotting. CD99 stabilized by clofarabine stays in solution at higher temperatures; (B) Graph represents band density of CD99 from panel a. Densities of bands were measured with ImageJ and analysis was done using GraphPad Prism. (C, D) MG63.3 OS and TC-71 ES cell lines were transiently transfected with control (black lines) or DCK siRNA (blue lines). DCK protein expression was confirmed by western blot. Reduced DCK expression resulted in complete resistance to clofarabine in OS cells but not in ES cells.