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. 2021 Jun 16;16(6):e0253170. doi: 10.1371/journal.pone.0253170

Fig 3. Clofarabine and CD99 antibodies cause specific protein phosphorylation in ES cell lines.

Fig 3

(A) STA-ET-7.2 cells treated with clofarabine and western blot analyses were done by phosphospecific antibodies. Phosphorylation of ERK1/2, MSK1/2 and CREB levels were increased with clofarabine treatment; (B) TC-71 cells treated with two different C99 antibodies (15μg/ml) and phosphorylation changes were analyzed by immunoblotting. Similar to clofarabine treatment, phosphorylation of ERK1/2, MSK1/2 and CREB were increased; (C, D) STA-ET-7.2 cells treated with clofarabine, CD 99 antibody and decreased phosphorylation changes for STAT3 and AMPKα1 were validated by western blotting; (E) A4573 cells treated with clofarabine show increased level of pMSK1/2 and main phosphorylated protein is pMSK2.