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. 2021 Jun 16;16(6):e0253170. doi: 10.1371/journal.pone.0253170

Fig 5. Phosphorylation changes after treatment for different ES and OS cell lines.

Fig 5

(A) Cytarabine’s effect on STA-ET.7.2 cells compared to the clofarabine treatment. Cells were treated with clofarabine, cytarabine or DMSO. Immunoblot analysis showed cytarabine did not alter phosphorylation of ERK1/2, MSK1/2 and CREB; (B) Immunoblot results of phosphorylation changes after clofarabine treatment of different ES cell lines; clofarabine caused increased phosphorylation of ERK1/2, MSK1/2 and CREB in all five ES cell lines; (C) Clofarabine treated OS cell lines did not show significant increase in phosphorylation of ERK1/2, MSK1/2 and CREB. Actin was used as the loading control for all experiments. (CLF: Clofarabine, CYT: Cytarabine).