Table 1. Comparison of culture systems.
| Name | Type | Classification | Vendor | V (mL) | SA (cm2) | SA:V | Cell type | SC/mL x106 | SC/cm2 x104 | Total SCs x106 | Td (hr-1) | Shear (dyne cm-2) | Source |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Packed Bed | PDMS Matrix | Immobilized | - | 110 | 2,800 | 25.5 | hP-MSC | 0.509 | 2.00 | 56 | 30.2 | 1–5 | [19] |
| Quantum | Hollow Fiber | Immobilized | TERUMOBCT | 1,440 | 21,000 | 14.6 | hAd-MSC | 0.167 | 1.14 | 240 | 34.1 | 0.3–0.7 | [20–23] |
| Mobius | STBR | Suspension | Millipore Sigma | 50,000 | 300,000 | 6 | hBM-MSC | 2.00 | 1.67 | 5,000 | 54.0 | 2–40 | [21,24,25] |
| Appliflex | Wave Bag | Suspension | Applikon | 1,500 | 7,360 | 4.91 | hAd-MSC | 0.190 | 3.87 | 285 | 31.2 | 0.1–0.5 | [6,26] |
| Mag 3 | Paddle | Suspension | PBS | 3,000 | - | - | hBM-MSC | 1.90 | - | 5,700 | 63.0 | - | [27] |
| Xpansion Multiplate | Parallel Plate | Immobilized | Pall | 1600 | 6,120 | 3.83 | hAd-MSC | 0.111 | 5.4 | 334 | 34.1 | 0.1 | [28,29] |
| iCellis | Random Fiber Matrix | Immobilized | Pall | 1000–5000 | 40,000 | 40 | hBM-MSC | 2.93 | 16 | - | 67.2 | 1–5 | [25,30,31] |
| Novel System | Lattice | Immobilized | - | 20 | 122 | 6.1 | hBM-MSC | 0.2 | 2 | 1.8 | 82 | 0.0029 | - |
Abbreviations: Volume (V), SA Surface Area (SA), Stem Cells (SC), Doubling Time (Td), Mesenchymal Stem Cell (MSC), Human Bone Marrow (hBM), Human Adipose Derived (hAd), Human Placental (hP).
Volume, available surfaces areas, cell types used, total stem cell yield normalized to volume and surface area, total overall yield, doubling time, and reported shear stress of various bioreactors for MSC culture.