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Young’s moduli of 30%, 40%, 50% and 60% MeOH-annealed silk films measured by AFM nanoindentation. Data was fit with linear elastic Hertzian contact model to approximate values of elastic moduli. A portion of the graph is magnified for better visualization of data for 30%, 40% and 50% films (data is represented as individual measurements with mean ± SD from three separate samples per condition. **p < 0.01, ****p < 0.0001).
