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. 2021 Feb 26;106(7):2057–2076. doi: 10.1210/clinem/dgab106

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Skeletal muscle mechanistic target of rapamycin (mTOR) phosphorylation status, presented as total mTOR protein during postexercise (A) and overnight (B) periods; mTOR phosphorylated at Ser2448 during postexercise (C) and overnight (D) periods; and the ratio of phosphorylated to total protein during postexercise (E) and overnight (F) periods following 300 unilateral eccentric knee extensor contractions (time = 0 hours) (damaged; black bars), while the contralateral leg rested (control; white bars). The postexercise period incorporated a work-matched bout of maximal concentric knee extensor contractions and ingestion of either a postexercise protein-polyphenol (PP; n = 9) or isocaloric maltodextrin placebo (PLA; n = 9) drink. The subsequent 9-hour overnight period began with ingestion of either a prebed PP (n = 9; black bars) or isocaloric maltodextrin PLA (n = 9; white bars) drink. α-tubulin was used as a loading control. Images obtained from a single representative participant in PLA. Data are presented as means, with error bars representing standard error. Statistical analysis was performed with separate 2-factor analysis of variance. *Main effect of time; ^#difference between damaged and control legs; ^†difference between PLA and PP; ^$decrease between 24 and 27 hours in PLA only. One symbol, P < 0.05; 2 symbols, P < 0.01; 3 symbols, P < 0.001.