Skip to main content
. 2021 Jun 16;7(25):eabf0797. doi: 10.1126/sciadv.abf0797

Fig. 1. Design and mode of function of the light-controlled viral transduction system.

Fig. 1

The OptoAAV system comprises (i) an engineered AAV-2 vector displaying PIF6 on its surface and containing mutations that ablate its natural tropism for HSPG (OptoAAV) and (ii) an adapter protein consisting of PhyB and a DARPin selectively binding to a specific cell surface protein. Illumination with 660-nm light induces the interaction of PhyB with PIF6 and thus the recruitment of the engineered OptoAAV to the cell surface resulting in transduction of the target cell.