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. 2021 May 6;20(6):e13370. doi: 10.1111/acel.13370

FIGURE 5.

FIGURE 5

PC4 promoted protein synthesis and decreased proteostasis by activated mTOR signalling. (a–e) Results and statistical analysis of mTOR signalling‐related protein including Rheb, P70S6K, S6RP, mTOR and their phosphorylated forms were detected by Western blot in 18‐month‐old PC4(+/+) or PC4(KI/KI) mice (a and b) and in 2BS cells at indicated passage (c and d) n = 3. (e–k) Results of mTOR signalling‐related protein (e and f), protein synthesis assessed by puromycin incorporation (g), proteostasis assessed by protein aggregation (h) and senescence‐associated indicators including protein level of P16 and P21 (i, n = 3), SA‐β‐gal (j, n = 3) and SASP (k, n = 4) in 2BS cells after vehicle or RAPA treatment for five consecutive passages. Rapamycin (RAPA, 0.1 μM), a classical mTOR inhibitor. For (e, f, g, i and j), n = 3. For (h), n = 5. For (k), n = 4. For (b), two‐tailed Student's t‐test; For (d), one‐way ANOVA; For (f–k), two‐way ANOVA; *p < 0.05