Figure 3.

Experimental setup for antigen cross-presenting assay. The ability of Vγ9Vδ2 T cells to cross-present antigens was tested as schematically outlined. Vγ9Vδ2 T cells were expanded for 9-11 days, as described in the method section. Next, long peptide or protein was added to wells with Vγ9Vδ2 T cells, followed by 24 h incubation, to allow antigen uptake and cross-presentation. Then, the Vγ9Vδ2 T cells were washed twice to remove excess long peptide or protein. Next, Vγ9Vδ2 T cells were transferred to the ELIPOT plate and antigen specific αβTCR T effector cells (Teff) were added. IFNγ secretion following specific target recognition was measured by IFNγ ELISPOT assay. Vγ9Vδ2 T cells alone were used as negative controls. For positive controls, short peptide was added to wells containing both Vγ9Vδ2 T cells and Teff leading to maximal recognition and associated cytokine secretion by Teff.Figure is created with Biorender.