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. 2021 Jun 2;8:674747. doi: 10.3389/fmolb.2021.674747

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Copyright © 2021 Hoyt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An 8-plex, 9-color tonsil composite image with respective channel monoplex images: Opal Polaris 480 = CD20 (purple), Opal 520 = CD8 (yellow), Opal 540 = PD-L1 (red), Opal 570 = FoxP3 (orange), Opal 620 = CD68 (blue), Opal 650 = PD-1 (green), Opal 690 = Ki67 (white), Opal Polaris 780 = PanCK (teal), and DAPI counterstain.