Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 2;8:674747. doi: 10.3389/fmolb.2021.674747

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Hoyt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Whole-slide MOTiF image of lung cancer FFPE tissue. (A) Markers stained for this 6-plex, 7-color assay include CD8 (yellow), PD-L1 (red), FoxP3 (orange), PD-1 (magenta), cytokeratin (cyan), and CD68 (green). White box indicates (B) selected area from whole tissue section image at 20× magnification highlighting the interactions between the immune system and the tumor (i.e., “hotspot”). The cellular composition and distribution reveal immune engagement with the tumor, evidenced by tumor-infiltrating lymphocytes (TILs), PD-L1+ macrophages, PD-L1− tumor cells, and an abundance of para-tumoral regulator T-cells and cytotoxic T-cells, including several that are PD-1+. Elucidating the interplay between these different cell types is key to understanding the variance in patient responsiveness to therapeutic treatments.