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. 2021 Jun 2;8:674747. doi: 10.3389/fmolb.2021.674747

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Copyright © 2021 Hoyt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of the BOND RX performance is captured by (A) running a CD20-Opal 520 monoplex assay across 30 serial sections. The percent coefficient of variation (%CV) is calculated by determining the mean expression of the top 20 brightest expressing cells across five matching annotations on all the serial sections. (B) Similarly, an optimized 6-plex, 7-color assay is also run on 15 serial sections, and the mean IF counts of the top 20 cells per marker are measured and the mean, standard deviation, and %CV are calculated.