Fig. 1. At-RSPHERE root commensals exhibit strain-specific variations to suppress flg22-mediated RGI in pWER::FLS2-GFP plants.
a, Phylogenetic tree showing the distribution of strains exhibiting RGI-suppressive activity. The outer rings represent root lengths of plants (aged 3 weeks) germinated on plates supplemented with 1 μM flg22 and individual strains (OD600 = 0.0005). n = 2 independent replicates. The threshold for suppressive activity is indicated by the red line, that is, root length > 2 cm. b, The impact of four independent five-member SynComs (Supplementary Table 1), which differed in RGI-suppressive activity, on flg22-mediated RGI. Plants (aged 2 weeks) were germinated on plates supplemented with 1 μM flg22 and the indicated SynCom. Shapes represent three (SynCom NS1 + S1) and four (SynCom NS2 + S2) independent replicates in b. The n values indicate the total number of biological samples. Different letters indicate statistical significance, determined using two-sided Dunn’s Kruskal–Wallis test (P < 0.05). The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within 1.5× the interquartile range.