Extended Data Fig. 10. MYB15 is a positive regulator of defense against specific commensals.
a,b, Expression of flg22-induced marker genes, PER5 and FRK1, in roots of 14-day-old pWER::FLS2-GFP plants upon treatment with the indicated SynCom. c, Expression of FRK1, MYB15 and PER5 of Col-0 plants 1 hr and 24 hr post flg22 treatment. Plants were precolonized by the indicated SynCom for two weeks. Expression patterns of WRKY28, WRKY30 and MYB15 in roots of 14-day-old (d) Col-0 and (e) pWER::FLS2-GFP transcriptome experiments. myb15-1 mutant enhances proliferation of Pseudomonas 401 (f) and Variovorax 434 (g). h, Expression of FRK1, PER5 and chitinase (AT2G43620) upon coinoculation between chitin (1 mg/ml) and SynComs at the indicated time points. Plants were precolonized by the indicated SynCom for two weeks. i, Individual strains or combined SynComs do not antagonize 401 growth in vitro. Individual strain was spotted on a background lawn of 401 and halo-of-inhibition (if any) was noted 5 dpi. n=total number of biological samples collected from three (a-b, g, 1 and 6 hr), four (c) or two biological replicates (g, 24 hr). Asterisks and different letters indicate statistical significance. One-sided student’s t-test (a-c), two-sided Dunn’s Kruskal Wallis (d-e), two-sided Dunnett’s test (f-g) and two-sided ANOVA (h) were used, p < 0.05). The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within the range of 1.5× the interquartile range.