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. 2021 May 24;7(6):814–825. doi: 10.1038/s41477-021-00920-2

Extended Data Fig. 2. Microbiota members modulate root growth inhibition (RGI) induced by Atpep1 and function quantitatively in community contexts.

Extended Data Fig. 2

a, Root lengths of WT and pepr1pepr2 mutants (left panel) or WT plants (right panel) inoculated with 1 μM Atpep1 alone or with individual strain. b, Caulobacter 342 exhibits differential ability to suppress flg22- or Atpep1-mediated RGI. c, Representative images of WT, fls2 and pWER::FLS2-GFP plants grown in natural Lausanne soil and their corresponding shoot fresh weight without any flg22 treatment. d, Root lengths of pWER::FLS2-GFP plants inoculated with different combination of non-suppressive and suppressive strains in the presence of 1 μM flg22. n=total number of biological samples collected from four (a), two (b), two (c) and three (d) biological replicates. Asterisks indicate statistical significance (two-sided Dunn’s Kruskal Wallis or two-sided Dunnett’s test, p < 0.05). The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within the range of 1.5× the interquartile range. e, Gel blot (Source ED Fig. 2e) showing the result of PCR genotyping of 569ΔhrcC generated by homologous recombination. Lane from left to right, GeneRuler 1 kb plus ladder, four individual colonies and control. f, Root lengths of pWER::FLS2-GFP plants coinoculated with flg22 and 569 or 569ΔhrcC.

Source data