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. 2021 May 24;7(6):814–825. doi: 10.1038/s41477-021-00920-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Schematic diagram showing how bacterial supernatants are collected. Root lengths of plants coinoculated with the supernatant of different bacteria and b, 1 μM flg22 (pWER::FLS2-GFP) or c, 1 μM Atpep1 (Col-0). All strains used in these experiments exhibited RGI suppressive activity as shown in Fig. 1. d, Root lengths of Col-0 plants treated with the supernatant of Janibacter 101 after different treatments shown in (a). e, Mass spectrometry analyses detecting the intact flg22 peptide (QRLSTGSRINSAKDDAAGLQIA [M + 4H] 568.8056) by estimating the peak area generated by Skyline. All strains used in this experiment exhibited suppressive activity on flg22-mediated RGI. n=total number of biological samples collected from three biological replicates (ED Fig. 3b–e). Asterisks or different letters indicate statistical significance (two-sided Dunn’s Kruskal Wallis or two-sided Dunnett’s test, p < 0.05). The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within the range of 1.5× the interquartile range.

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