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. 2021 May 24;7(6):814–825. doi: 10.1038/s41477-021-00920-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a-c, Constrained coordination of the microbial profile of Col-0 root samples showing the effect of flg22 and SynCom. Ellipses correspond to Gaussian distributions fitted to each cluster (95% confidence interval). p-values next to ellipses indicate statistical significance based on a PERMANOVA test between untreated and flg22-treated samples of each SynCom (permutation = 999, p < 0.05). d-f, Relative abundance of strains upon flg22 treatment. Experiment 1: (a,d); experiment 2: (b,e); experiment 3: (c,f). The corresponding strains used in each SynCom were indicated on top of (d-f). These experiments were conducted using different SynComs and repeated twice with consistent result. Values in bracket are eigenvalues explained by the Principal Component (PC). Colors indicate SynComs used and shapes indicate flg22 treatment. g, Simpson diversity index of the corresponding experiments performed with pWER::FLS2-GFP plants. n=total number of biological samples collected from three biological replicates. Asterisks indicate statistical significance (two-sided ANOVA, p < 0.05) and the p values are provided on top of each graph. The box plots centre on the median and extend to the 25th and 75th percentiles, and the whiskers extend to the furthest point within the range of 1.5× the interquartile range.