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. 2021 Jun 1;24(6):102678. doi: 10.1016/j.isci.2021.102678

Figure 6.

Figure 6

MSR1 interacts with VSV glycoprotein G via its extracellular domains

(A–C) Binding of MSR1 to VSV virions. FLAG-MSR1 (human, 1-end) and its fragments (aa1-80, 51-end) were expressed in HEK293T cells and immunoprecipitated (IP) using anti-FLAG antibody-coated magnetic beads, which were then incubated with intact VSV-GFP virions (4°C for 2 h). The bound virions were eluted for (A) immunoblotting (IB) with a mouse monoclonal anti-FLAG and rabbit anti-VSV-G antibody

(B and C) Re-infection in Vero cells for 12 h followed by detection of (B) VSV-GFP by fluorescence microscopy and (C) VSV-G protein level by immunoblotting. Mock: no virion control.

(D) co-IP of FLAG-MSR1 and its fragments with VSV-G in HEK293T cells transfected with plasmids. WCE: whole-cell extraction. Arrows point to the faint protein bands.