Fig. 3.

Frequency-dependent contractions by EFS, and effects of NF023 (100 μM), PPADS (50 μM), tetrodotoxin (TTX) (1 μM), and tamsulosin (300 nM) in human prostate tissues. Construction of frequency response curves was started 30 min following the administration of antagonists or TTX, or of equivalent amounts of solvents in controls. Shown are means ± SD from four independent series, each with prostate tissues from n = 7 (a), n = 8 (b), n = 5 (c), or n = 5 (d) different patients, where tissue from each prostate was allocated to the control and antagonist groups in each single experiment