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Effect of detergent on bacterial composition. Colonic biopsies (~3 mm) from 2 patients were isolated with our protocol using different detergents and concentrations. The bacterial signal for Firmicutes, Bacteroidetes, Actinobacteria, and γ‐Proteobacteria was calibrated with the universal 16S rRNA signal of the same patient (ΔCt) and was compared to PBS sample of the same patient (ΔΔCt). The difference to PBS was plotted
